Fig. 2. Intracellular localization of GFP-tagged Pin1 and GFP-tagged
mutant proteins and their localization in nuclear speckles.
A, HeLa cells transiently transfected with GFP, GFP-Pin1
(Pin1), GFP-Pin-C113A (C113A),
GFP-Pin1-isomerase domain (PPIase), GFP-Pin1-WW domain
(WW), and GFP-Pin1-R68,69L (R68,69L) were
analyzed for the localization of the tagged proteins. B,
colocalization of Pin1 and the Pin1-WW domain with the spliceosome
assembly factor, SC-35. HeLa cells transiently transfected with
GFP-Pin1 (Pin1), or GFP-WW domain (WW),
were inspected for the intracellular localization of GFP, the SC-35
staining (Anti SC-35), and the superimposition of
the individual images (Merge).