Table 1 Catalytic efficiency
(kcat/Km) of Pin1 and
Pin1 mutants
[M-1s-1]a
| Substrate |
Pin1 |
PPIase |
C113A |
R68L |
R69L |
R68,69L |
|
| Ac-AApSPR-pNA |
3.1 x 106 |
1.6 x 107 |
1.1 x 103 |
1.4 x 106 |
3.9 x 104 |
7.0 x 103 |
| Ac-AASPR-pNA |
2.9 x 103 |
n.d. |
0.9 x 103 |
2.7 x 103 |
2.9 x 103 |
2.5 x 103 |
|
a The catalytic efficiency of
the different proteins was calculated at a rate constant
(kobs.) of 0.012 s-1 except for the
mutant Pin1-C113A, with which the maximum rate constant (0.005
s-1) was used for the calculations.