Fig. 3. Pin1 is essential for tumor cell survival. A, HeLa, 293,
and SK-N-AS-cells, transiently transfected with GFP
(GFP), GFP-Pin1 (Pin1), and GFP-Pin1
antisense (Pin1-AS) were analyzed for the frequency of
apoptotic nuclei 48 h after transfection. B,
HeLa cells, transiently transfected with GFP, GFP-tagged Pin1, or the
indicated GFP-tagged mutant proteins, were analyzed for the frequency
of apoptotic nuclei 72 h after transfection. A and
B, average of three independent experiments is shown;
bars, SE. C, juglone induces apoptosis in
human tumor cells. Flow cytometric DNA analysis of HeLa cells grown for
72 h in the presence of the indicated concentration of juglone.
